PLSC 631 - Intermediate Genetics

Genomic Analysis Quiz

1. Flourescence in situ hybridization has also been called chromosome painting? Suggest a reason why you think this term is appropriate for FISH.

2. Compare and contrast RFLPs, VNTRs and microsatellites.

3. You are collaborating with a cell biologist who has developed four human-rodent hybrid cell lines. Your analysis of the enzyme activities of these four lines revealed the enzyme issel was active in cell lines A and C, enzyme thompson was active in line D, enzyme jones was active in all lines, and enzyme english was active in line B. From the table below determine upon which chromosome these enzymes are located. (The "+" symbol means the chromosome is present in the cell line, and "-" means the chromosome is missing in the line.)

Human Chromosome
Hybrid Colony 1 2 3 4 5 6 7 8
A + + - + - - - +
B + + + + - + - -
C + + - - - + + +
D + - - - + - + -

4. A series of eight STS markers were scored on six YACs. The results are shown in the table below where a "+" means the marker was detected on the YAC, and "-" means the marker was not detected on the YAC. From this information: a. determine the order of the STS markers; and b. order the YACs to form a contig.

STS Marker
YAC A D E F G H X Z
1 - - - + + - - -
2 - - + - - + + +
3 - + - + + - - -
4 - - - + - + - -
5 + - + - - - - +
6 + - - - - - - +